In the video we see a single U2OS cell expressing freely diffusible mNeonGreen treated with SASR-CoV-2 VLP-Wuhan ( E,S, M&M-mCherry). On the left side is the raw image of the cell, in the middle is the cell with transparent cell volume and on the right is the cell with opaque volume.

U2OS

U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.

mNeonGreen cell labelling,

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

No treatment