A complete understanding of SARS-CoV-2 internalization into cells requires accurate measurement of the kinetics for the molecular events involved.

Here, we present live-cell imaging of binding, entry, endosome ingression, acidification, and microtubule transport of fluorescently-labeled SARS-CoV-2 virus-like particle (VLP) into cells. We have imaged this process in multiple cell lines using VLPs representative of multiple SARS-CoV-2 strains.

This database allows the user to select the experiment they wish to view based on cell line (U2OS, Vero E6, and A549) or SARS-CoV-2 VLP variant (Wuhan, Omicron, and Del-1), which can be selected on the top left corner of the page.

VLP composition and nomenclature:
If otherwise specified the VLPs contain the four structural proteins: envelope protein (E), membrane protein (M), nucleocapsid pronein (N), spike pronein (S). Depending on the experimental setting some of the proteins are abelled with fluorescent protein markers such as: pHluorin, mCherry and EGFP, e.g. labelling of the membrane protein (M) with mCherry is denoted as M-mCherry. Additionally, if there is viral RNA in the VLPs it is denoted as R (T20 RNA, as demonstrated in Syed, A. M. et al.)

The database was funded by Ministry of Education and Science via the The National Research Infrastructure Roadmap ДО1-166.