Internalization of VLP-Wuhan:(E, S, M&M-mCherry) into cells expressing ACE-2-NeonGreen. The VLPs are red and the ACE2 receptor is green in the video. We observed adherence of the VLPs to the cell membrane, often on the filopodia.

U2OS

U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.

ACE2-NeonGreen cell labelling,

The ACE2 receptor is tagged with NeonGreen and this shows the margins of a cell under a microscope.

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

No treatment