VeroE6 cells expressing Dynamin-1-GFP treated with  Dynamin inhibitor (Dynole 34-2) and consecutively with VLP-Wuhan:(E, S, M&M-mCherry). After Dynol 34-2 treatment, VLPs did bind to the cell surface but did not enter them.

VeroE6

VeroE6 cells were isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.)

No cell labelling,

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

Dynol34-2

Cell inhibitor preventing SARS-CoV-2 particles to enter the cells.