U2OS cells which express freely diffusible NeonGreen are treated with VLP-Wuhan:(E, S, M&M-mCherry). We can see the internalization of the VLPs over the course of the videos.

U2OS

U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.

mNeonGreen cell labelling,

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

No treatment