Internalization in cells, in which tubulin is labelled

Internalization of VLP-Wuhan ( E,S, M&M-mCherry) into VeroE6 cells treated with Aberior Live 610 (cabazitaxel conjugated with LIVE 610 dye), a tubulin marker. We can see that the particles move through the tubulin network once inside the cells.

 

Cell

VeroE6

VeroE6 cells were isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.)

Tubulin-Aberior, Tubullin tagged with Aberrior 610 Live dye allowing us to track the tubulin network in the cell and thus the cell itself.

Virus

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

Treatment

No treatment