Internalization in cells expressing Rab-5-GFP

Vero E6 cells expressing Rab-5-GFP, which marks early endosomes, treated with VLP-Wuhan:(E, S, M&M-mCherry). There is no colocalization of the VLPs with the early endosomes. 

 

Cell

VeroE6

VeroE6 cells were isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.)

Rab5-GFP, Rab5 tagged with GFP allowing us to track EarlyEndosomes and thus visualize the cells.

Virus

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

Treatment

No treatment