Internalization in cells treated with LysoTracker

Vero E6 cells pre-incubated with VLP-Wuhan:(E, S, M&M-mCherry) and then treated with LysoTracker, which allows us to track acidic vesicles. VLPs colocalized with the stained acidic vesicles.

 

Cell

VeroE6

VeroE6 cells were isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.)

LysoTracker, A dye for acidic vesicles allowing us to track them in the cell and thus visualize it.

Virus

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

Treatment

No treatment