VLPs pre-incubated with anti-SARS-CoV-2 antibodies

Cells inoculated with  VLP-Wuhan ( E,S, M&M-mCherry), pre-treated with an Anti-SARS-CoV-2 Spike S1 (CR3022 clone) antibody. Antibody pre-incubation precipitated most of the VLPs, preventing cell entry by effectively reducing their free concentration.

 

Cell

VeroE6

VeroE6 cells were isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.)

GFP labelling,

GFP expressed in a cell.

Virus

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-mCherry, Part of the M protein of the particle is tagged with mCherry. This allows us to track the membrane of the particle under the microscope.

Treatment

Antibody

Antibody that leads to the congregation of SARS-CoV-2.