Internalization of SARS-CoV-2-Omicron:(E, S, N&N-eGFP, M&M-mCherry, T20 RNA) VLPs into A549 cells. These cells contain M-mCherry (Red) and N-EGFP (Green). This allows us to simultaneously track the dynamics of the membrane together with its nucleocapsid one. Initially, the particle emits yellow color because of the two overlaping signals, but during nucleocapsid from the VLP, the N-EGFP signal slowly decreases and the particle emits only red signal and increases its movement.

A549

A549 cells are lung carcinoma epithelial cells.

No cell labelling,

SASR-CoV-2 VLPs Omicron

SARS-CoV-2 virus-like particles of the Omicron (Omicron BA.1) variant. The particles contain 39 mutations compared to the WT (Wuhan) strain of the virus.

M-pHluorin N-EGFP, Part of the M protein of the particle is tagged with mCherry and part of N protein is tagged with EGFP. This allows us to track the membrane of the particle simultaneously with its nucleocapsid under the microscope.

No treatment